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PubMed · 7723751

[Use of continuous stacking hybridization in sequencing using modified oligonucleotide matrices].

Iu N Lysov·A A Chernyĭ·A A Balaev·F N Gnuchev·K L Beattie·A D Mirzabekov
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Abstract

The opportunity of DNA sequencing by hybridization with oligonucleotide matrix (SHOM) with simultaneously use continuous stacking hybridization and gapped-matrices is considered. The analysis of reconstruction efficiency for various combinations matrices and l-oligonucleotides libraries were made. In most cases combine use of continuous stacking hybridization and gapped matrices permits to decrease the number of additional stacking hybridization twice without lost of efficiency.

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Nucleic Acid HybridizationOligonucleotidesSequence Analysis, DNATemplates, GeneticEnglish AbstractJournal Article

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Iu N Lysov, A A Chernyĭ, A A Balaev, F N Gnuchev, K L Beattie, A D Mirzabekov. [Use of continuous stacking hybridization in sequencing using modified oligonucleotide matrices].. https://pubmed.ncbi.nlm.nih.gov/7723751/

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