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Generation and characterization of an ordered lambda clone array for the 460-kb region surrounding the murine Xist sequence.

C Rougeulle·L Colleaux·B Dujon·P Avner
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Abstract

The Xist sequence has several characteristics that make it a potential candidate for the X-inactivation center. To investigate the role of Xist and adjacent sequences lying within the X-inactivation center candidate region, a 460-kb region surrounding the murine Xist sequence has been arrayed in lambda contigs with a combination of IRS-PCR-based hybridization and YAC fragmentation. The orientation of the Xist sequence in relation to the telomere and centromere of the X Chromosome (Chr) has been established with this contig and shown to be inverted compared to that in human.

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AnimalsBacteriophage lambdaBase SequenceChromosomes, Artificial, YeastCloning, MolecularDosage Compensation, GeneticFemaleMaleMiceMice, Inbred C3HMice, TransgenicMolecular Sequence DataOligodeoxyribonucleotidesPolymerase Chain ReactionRNA, Long NoncodingRNA, UntranslatedTranscription FactorsX ChromosomeJournal ArticleResearch Support, Non-U.S. Gov't

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C Rougeulle, L Colleaux, B Dujon, P Avner. 1994. Generation and characterization of an ordered lambda clone array for the 460-kb region surrounding the murine Xist sequence.. https://doi.org/10.1007/bf00357001

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