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PubMed · 7727132

High-performance membrane chromatography.

B G Belenkii·V G Malt'sev
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Abstract

In gradient chromatography for proteins migrating along the chromatographic column, the critical distance X0 has been shown to exist at which the separation of zones is at a maximum and band spreading is at a minimum. With steep gradients and small elution velocity, the column length may be reduced to the level of membrane thickness--about one millimeter. The peculiarities of this novel separation method for proteins, high-performance membrane chromatography (HPMC), are discussed and stepwise elution is shown to be especially effective. HPMC combines the advantages of membrane technology and high-performance liquid chromatography, and avoids their drawbacks.

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Chromatography, High Pressure LiquidFerritinsMembranes, ArtificialProteinsJournal Article

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B G Belenkii, V G Malt'sev. 1995. High-performance membrane chromatography.. https://pubmed.ncbi.nlm.nih.gov/7727132/

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