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PubMed · 7731794

Coding sequence composition flanking either signal element alters V(D)J recombination efficiency.

N V Boubnov·Z P Wills·D T Weaver
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Abstract

Lymphoid V(D)J rearrangement is targeted by recombination signal sequences (RSS) bordering V, D or J exons. We demonstrate that the DNA composition of flanking coding positions, particularly poly(A) or poly(T) stretches at one or both RSS, diminishes V(D)J recombination up to 100-fold. Positionally correct cleavages occur in the inhibited reactions, since the junctions formed show the same frequency of precision as uninhibited reactions. Open/shut cleavage/rejoining is not increased at a normal RSS in substrates containing inhibitory A/T homopolymers versus random sequence at a second RSS. Thus recombinase action at both cleavage sites is severely disrupted by modified coding sequences.

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B-LymphocytesBase SequenceDNA NucleotidyltransferasesGene Rearrangement, B-LymphocyteIntegrasesMolecular Sequence DataOligodeoxyribonucleotidesRecombinasesRecombination, GeneticTemplates, GeneticTumor Cells, CulturedJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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N V Boubnov, Z P Wills, D T Weaver. 1995-03-25. Coding sequence composition flanking either signal element alters V(D)J recombination efficiency.. https://doi.org/10.1093/nar%2F23.6.1060

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