Higher reproducibility of morphometric analysis over the counting method for interphase AgNOR quantification.

D Trerè; M Migaldi; G P Trentini

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PubMed · 7734412

Higher reproducibility of morphometric analysis over the counting method for interphase AgNOR quantification.

D Trerè·M Migaldi·G P Trentini

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Abstract

In a series of 40 breast carcinomas, the reproducibility of two different methods for interphase AgNOR quantification was evaluated. Two operators independently defined on each slide the interphase AgNOR quantity both by measuring the area of the silver-stained structures using image cytometry and counting the AgNOR number directly at the microscope. The correlation between the values obtained by the two observers was statistically significant, but the correlation coefficient between AgNOR areas (r = 0.79; P < 0.001) was greater than that between AgNOR numbers (r = 0.38; P = 0.014). On the other hand, when interphase AgNOR area and number values obtained by each observer were compared, no significant correlation was found. This study has demonstrated that the two different methods for interphase AgNOR quantification are not comparable, and that morphometric analysis is more objective and reproducible than the counting method.

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Humans Image Processing, Computer-Assisted Interphase Nucleolus Organizer Region Observer Variation Reproducibility of Results Silver Staining Comparative Study Journal Article Research Support, Non-U.S. Gov't

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D Trerè, M Migaldi, G P Trentini. 1995. Higher reproducibility of morphometric analysis over the counting method for interphase AgNOR quantification.. https://pubmed.ncbi.nlm.nih.gov/7734412/

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