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Mycoplasmas regulate HIV-LTR-dependent gene expression.

R Nir-Paz·S Israel·A Honigman·I Kahane
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Abstract

Mycoplasmas have been incriminated in setting the stage for HIV infection and full-blown AIDS. We tested the possible involvement of mycoplasmas in activation of HIV. Two cell lines, 293 fibroblasts and Jurkat CD4+ T-cells, transfected with plasmids harboring a transcription fusion construct between HIV-long terminal repeat (HIV-LTR) and either luc or cat genes, were infected with several mycoplasmas (M. fermentans; M. penetrans, M. pirum and Ureaplasma urealyticum) and the reporter gene expression was monitored. The data presented here suggest that mycoplasmas, and specifically their membranes, play a role in the activation of HIV-LTR mediated transcription.

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CD4-Positive T-LymphocytesCell LineFibroblastsGene Expression Regulation, ViralGenes, ReporterHIV InfectionsHIV Long Terminal RepeatHumansMycoplasmaMycoplasma InfectionsJournal ArticleResearch Support, Non-U.S. Gov't

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R Nir-Paz, S Israel, A Honigman, I Kahane. 1995-04-15. Mycoplasmas regulate HIV-LTR-dependent gene expression.. https://doi.org/10.1111/j.1574-6968.1995.tb07501.x

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