Larger scale multipin peptide synthesis.

N J Maeji; A M Bray; R M Valerio; W Wang

PubMed · 7756752

Larger scale multipin peptide synthesis.

Abstract

The multipin peptide synthesis approach originated as an immunological tool for epitope mapping. However, continuing evolution of the basic technology has allowed synthesis at scales up to 10 mumol per pin. At this loading, the methodology can no longer be considered just a screening tool. The overall synthesis efficiency of this approach was assessed by the synthesis of 2913 different peptides having little or no sequence homology and ranging up to a 46-mer in length. High performance liquid chromatography analysis of the crude peptides indicates overall quality of synthesis was high. The method is suitable for multi-milligram synthesis of peptides without sacrificing any of the inherent advantages of the 96-well format.

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Amino Acid Sequence Chromatography, High Pressure Liquid Molecular Sequence Data Peptides Journal Article

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N J Maeji, A M Bray, R M Valerio, W Wang. Larger scale multipin peptide synthesis.. https://pubmed.ncbi.nlm.nih.gov/7756752/

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