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PubMed · 7764446

On-line immunoanalysis for bioprocess control.

C Middendorf·B Schulze·R Freitag·T Scheper·M Howaldt·H Hoffmann
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Abstract

Immunoanalytical techniques such as ELISA are often used for the detection of proteins produced in cultivation processes. Owing to the difficulty of automating of the time-consuming traditional ELISA, there is an intense demand for a suitable on-line monitoring method. Combining well-known immunoassays with the FIA technique, we present the heterogeneous and the turbidimetric immuno-FIA methods. The following proteins were investigated with these FIA methods: thermostable pullulanase, IgG, antithrombin III, and recombinant tissue-type plasminogen activator. In the cases of pullulanase and monoclonal mouse IgG, the turbidimetric immuno-FIA was used for on-line analysis of the cultivation process. Results are presented here to demonstrate the effectiveness and application of these immunoanalysis.

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Antithrombin IIIBiosensing TechniquesEnzyme-Linked Immunosorbent AssayGlycoside HydrolasesImmunoassayImmunoenzyme TechniquesImmunoglobulin GNephelometry and TurbidimetryRecombinant ProteinsTissue Plasminogen ActivatorComparative StudyJournal Article

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C Middendorf, B Schulze, R Freitag, T Scheper, M Howaldt, H Hoffmann. 1993. On-line immunoanalysis for bioprocess control.. https://doi.org/10.1016/0168-1656(93)90083-y

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