A host-vector system for a cellulose-producing Acetobacter strain.

N Tonouchi; T Tsuchida; F Yoshinaga; S Horinouchi; T Beppu

doi:10.1271/bbb.58.1899

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A host-vector system for a cellulose-producing Acetobacter strain.

N Tonouchi·T Tsuchida·F Yoshinaga·S Horinouchi·T Beppu

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Abstract

An indigenous plasmid, named pAH4, was detected in a cellulose-producing Acetobacter strain. This plasmid, consisting of 4002 bp, contained an AT-rich region and encoded several open reading frames, as deduced by the complete nucleotide sequence. One of the putative open reading frames showed homology with replication proteins of other plasmids. A shuttle vector of Escherichia coli and this strain was constructed by connecting pAH4 to pUC18. Electroporation of the shuttle vector into the strain yielded 1.7 x 10(5) ampicillin resistant transformants per microgram DNA. The shuttle plasmid was very stably maintained in the strain.

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Acetobacter Amino Acid Sequence Ampicillin Resistance Base Sequence Cellulose DNA, Bacterial Escherichia coli Genetic Vectors Molecular Sequence Data Open Reading Frames Plasmids Sequence Homology, Nucleic Acid Journal Article

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N Tonouchi, T Tsuchida, F Yoshinaga, S Horinouchi, T Beppu. 1994. A host-vector system for a cellulose-producing Acetobacter strain.. https://doi.org/10.1271/bbb.58.1899

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