Prolonged cell-free protein synthesis in a batch system using wheat germ extract.

Y Kawarasaki; H Nakano; T Yamane

doi:10.1271/bbb.58.1911

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Prolonged cell-free protein synthesis in a batch system using wheat germ extract.

Y Kawarasaki·H Nakano·T Yamane

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Abstract

Reaction conditions of cell-free protein synthesis using wheat germ extract were examined to prolong the period of protein synthesis in a batch reaction. By optimizing conditions for ATP regeneration system involved in the cell-free system, protein synthesis continued about 4 hours, so that about 17 micrograms dihydrofolate reductase protein was obtained in 1 ml of a reaction mixture. It suggests that maintaining ATP concentration is the primary requirement for long-life cell-free protein synthesis.

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Adenosine Triphosphate Cell-Free System Guanosine Triphosphate Leucine Plant Extracts Plant Proteins RNA, Messenger Tetrahydrofolate Dehydrogenase Triticum Journal Article Research Support, Non-U.S. Gov't

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Y Kawarasaki, H Nakano, T Yamane. 1994. Prolonged cell-free protein synthesis in a batch system using wheat germ extract.. https://doi.org/10.1271/bbb.58.1911

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