IS292: a novel insertion element from Agrobacterium.

C Ponsonnet; P Normand; G Pilate; X Nesme

doi:10.1099/13500872-141-4-853

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IS292: a novel insertion element from Agrobacterium.

C Ponsonnet·P Normand·G Pilate·X Nesme

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Abstract

A new insertion sequence, IS292, located in the 6b gene of a nopaline-type Agrobacterium strain (X88-292) isolated from poplar was identified and sequenced. IS292 is 2494 bp long, has 21 bp inverted terminal repeats with two mismatches, and generates 10 bp direct repeats upon integration. No sequence similarity was found between IS292 and other insertion elements associated with Agrobacterium, but it shows strong similarity with ISR/1 from Rhizobium leguminosarum bv. viciae. The occurrence of IS292-like sequences in various Agrobacterium isolates, especially different Agrobacterium strains isolated from the same biotope, was demonstrated by DNA hybridization.

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Amino Acid Sequence Bacterial Proteins Base Sequence DNA Primers DNA Transposable Elements DNA, Bacterial Genes, Bacterial Molecular Sequence Data Restriction Mapping Rhizobium Sequence Homology, Amino Acid Species Specificity Comparative Study Journal Article Research Support, Non-U.S. Gov't

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C Ponsonnet, P Normand, G Pilate, X Nesme. 1995. IS292: a novel insertion element from Agrobacterium.. https://doi.org/10.1099/13500872-141-4-853

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