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PubMed · 7785784

A microtiter plate-based high-throughput DNA purification method.

K Wang·L Gan·C Boysen·L Hood
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Abstract

A fast, reliable, inexpensive, and high-throughput method to purify DNA has been developed. It is based on DNA amplification by polymerase chain reaction utilizing a mini spin-column made from a 96-well membrane-bottomed assay plate. With this method, 50% of the DNA is recovered routinely using Sephacryl-500HR as filtration media. Purified DNA can then be used in various enzymatic manipulations, such as sequencing and hybridization. This provides an economical alternative protocol for routine large-scale purification of DNA templates for sequencing or other enzymatic manipulations.

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Base SequenceChromatography, GelColoring AgentsDNADNA PrimersFluorescenceMembranes, ArtificialMolecular Sequence DataNucleic Acid HybridizationPolymerase Chain ReactionSequence Analysis, DNATrypsinogenJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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K Wang, L Gan, C Boysen, L Hood. 1995-03-20. A microtiter plate-based high-throughput DNA purification method.. https://doi.org/10.1006/abio.1995.1195

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