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PubMed · 7787043

Fluorescence lifetime-based sensing in tissues: a computational study.

C L Hutchinson·J R Lakowicz·E M Sevick-Muraca
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Abstract

We have numerically solved the photon diffusion equation to predict the distribution of light in a tissue model system with a uniform concentration of fluorophore. Our results show that time-dependent measurements of light propagation can be used to monitor the fluorescent lifetimes of a uniformly distributed fluorophore in tissues. With proper referencing, frequency-domain measurements of phase-shift, theta, may allow quantitation of fluorescent lifetimes, tau, independent of changes in the local absorption and scattering properties. These results point to a new approach for noninvasive diagnostic monitoring through quantitation of fluorescent lifetime, tau, when the lifetime of the fluorophore is comparable with photon migration times.

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Biophysical PhenomenaBiophysicsBiosensing TechniquesDiffusionFluorescenceFluorescent DyesLightModels, BiologicalPhotonsScattering, RadiationTissue DistributionJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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C L Hutchinson, J R Lakowicz, E M Sevick-Muraca. 1995. Fluorescence lifetime-based sensing in tissues: a computational study.. https://doi.org/10.1016/s0006-3495(95)80330-9

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