Optical mapping of lambda bacteriophage clones using restriction endonucleases.

X Meng; K Benson; K Chada; E J Huff; D C Schwartz

doi:10.1038/ng0495-432

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Optical mapping of lambda bacteriophage clones using restriction endonucleases.

X Meng·K Benson·K Chada·E J Huff·D C Schwartz

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Abstract

Optical mapping is an emerging single molecule approach for the rapid generation of ordered restriction maps, using fluorescence microscopy. We have improved the size resolution of optical mapping by imaging individual DNA molecules elongated and fixed onto derivatized glass surfaces. Averaged fluorescence intensity and apparent length measurements accurately determined the mass of restriction fragments 800 basepairs long. We have used optical mapping to create ordered restriction maps for lambda clones derived from the mouse pygmy locus.

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Animals Bacteriophage lambda Chromosomes, Artificial, Yeast Cloning, Molecular DNA Electrophoresis, Gel, Pulsed-Field Glass Mice Microscopy, Fluorescence Molecular Weight Optics and Photonics Polylysine Restriction Mapping Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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X Meng, K Benson, K Chada, E J Huff, D C Schwartz. 1995. Optical mapping of lambda bacteriophage clones using restriction endonucleases.. https://doi.org/10.1038/ng0495-432

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