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A global approach for contig construction.

A Gleizes·A Hénaut
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Abstract

A program for assembling sequences by using a global approach has been developed. By successive steps, a more and more precise classification of DNA fragments permits the positioning of the sequences on the contig; after having detected the pairs of overlapping sequences, groups are formed such that all sequences in a group overlap. Sequences common to several groups enable the groups to be ordered in a series. Ambiguities in the order of groups can arise at this stage, due to the presence of repeated fragments; different solutions are then proposed. Putting the groups into order leads to a preclassification of sequences. The fragments are then aligned by group, by searching for words common to all sequences in the group, and using an algorithm of dynamic programming. A detailed example on a set of nine sequences accompanies the description of the method.

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AlgorithmsBase SequenceDNADNA, ComplementaryGenetic TechniquesMolecular Sequence DataSequence AlignmentSequence Analysis, DNASoftwareJournal Article

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BibTeXRIS

A Gleizes, A Hénaut. 1994. A global approach for contig construction.. https://doi.org/10.1093/bioinformatics%2F10.4.401

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