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Functional differences between L- and T-plastin isoforms.

M Arpin·E Friederich·M Algrain·F Vernel·D Louvard
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Abstract

Fimbrins/plastins are a family of highly conserved actin-bundling proteins. They are present in all eukaryotic cells including yeast, but each isoform displays a remarkable tissue specificity. T-plastin is normally found in epithelial and mesenchymal cells while L-plastin is present in hematopoietic cells. However, L-plastin has been also found in tumor cells of non-hematopoietic origin (Lin, C.-S., R. H. Aebersold, S. B. Kent, M. Varma, and J. Leavitt. 1988. Mol. Cell. Biol. 8:4659-4668; Lin, C.-S., R. H. Aebersold, and J. Leavitt. 1990. Mol. Cell. Biol. 10: 1818-1821). To learn more about the biological significance of their tissue specificity, we have overproduced the T- and L-plastin isoforms in a fibroblast-like cell line, CV-1, and in a polarized epithelial cell line, LLC-PK1. In CV-1 cells, overproduction of T- and L-plastins induces cell rounding and a concomitant reorganization of actin stress fibers into geodesic structures. L-plastin remains associated with microfilaments while T-plastin is almost completely extracted after treatment of the cells with non-ionic detergent. In LLC-PK1 cells, T-plastin induces shape changes in microvilli and remains associated with microvillar actin filaments after detergent extraction while L-plastin has no effect on these structures and is completely extracted. The effect of T-plastin on the organization of microvilli differs from that of villin, another actin-bundling protein. Our experiments indicate that these two isoforms play differing roles in actin filament organization, and do so in a cell type-specific fashion. Thus it is likely that these plastin isoforms play fundamentally different roles in cell function.

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Actin CytoskeletonActinsAmino Acid SequenceAnimalsBase SequenceCarrier ProteinsCell AdhesionCell PolarityCells, CulturedCytoskeletonEpithelial CellsEpitheliumFibroblastsHumansImmunohistochemistryMembrane GlycoproteinsMicrofilament ProteinsMicrovilliMolecular Sequence DataPhosphoproteinsRecombinant ProteinsTransfectionComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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M Arpin, E Friederich, M Algrain, F Vernel, D Louvard. 1994. Functional differences between L- and T-plastin isoforms.. https://doi.org/10.1083/jcb.127.6.1995

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