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PubMed · 7807713

Universal probe system for DNA fingerprinting.

Y Tamaki·T Kishida·M Fukuda
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Abstract

A universal probe system is a combination of an unlabeled primary probe that has been inserted in a cloning vector and a labeled secondary probe that is specific to the vector. This system is time- and labor-saving in that one does not need to label probes every time one uses them, so long as they are inserted in the same vector. As a first step in the preparation of a universal probe system for DNA fingerprinting, we isolated multi-locus probes from a subgenomic library that had been constructed by insertion into the phagemid pUC118 of 1-2 kb fragments of DNA from human myeloma cells. Next, we isolated single-stranded DNAs of recombinant phagemids, and hybridized them with Southern blots of DNAs from mother-child-father trios or unrelated individuals. As with double-stranded DNA probes, we were able to detect DNA fingerprints, with a commercially available, alkaline phosphatase-labeled secondary probe or a digoxigenin-labeled universal sequencing primer.

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DNA FingerprintingDNA ProbesGenomic LibraryHumansJournal Article

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Y Tamaki, T Kishida, M Fukuda. 1994. Universal probe system for DNA fingerprinting.. https://pubmed.ncbi.nlm.nih.gov/7807713/

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