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PubMed · 7809166

Human thrombopoietin: gene structure, cDNA sequence, expression, and chromosomal localization.

D C Foster·C A Sprecher·F J Grant·J M Kramer·J L Kuijper·R D Holly·T E Whitmore·M D Heipel·L A Bell·A F Ching
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Abstract

Thrombopoietin (TPO), a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Recently we have isolated a cDNA encoding a ligand for the murine c-mpl protooncogene and shown it to be TPO. By employing a murine cDNA probe, we have isolated a gene encoding human TPO from a human genomic library. The TPO locus spans over 6 kb and has a structure similar to that of the erythropoietin gene (EPO). Southern blot analysis of human genomic DNA reveals a hybridization pattern consistent with a single gene locus. The locus was mapped by in situ hybridization of metaphase chromosome preparations to chromosome 3q26-27, a site where a number of chromosomal abnormalities associated with thrombocythemia in cases of acute myeloid leukemia have been mapped. A human TPO cDNA was isolated by PCR from kidney mRNA. The cDNA encodes a protein with 80% identity to previously described murine TPO and is capable of initiating a proliferative signal to murine interleukin 3-dependent BaF3 cells expressing the murine or human TPO receptor.

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Amino Acid SequenceBase SequenceChromosomes, Human, Pair 3DNA PrimersDNA, ComplementaryGene ExpressionGenesHumansIn Situ Hybridization, FluorescenceMegakaryocytesMolecular Sequence DataNeoplasm ProteinsProto-Oncogene ProteinsRNA, MessengerReceptors, CytokineReceptors, ImmunologicReceptors, ThrombopoietinRecombinant ProteinsThrombopoietinJournal Article

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D C Foster, C A Sprecher, F J Grant, J M Kramer, J L Kuijper, R D Holly, T E Whitmore, M D Heipel, L A Bell, A F Ching. 1994-12-20. Human thrombopoietin: gene structure, cDNA sequence, expression, and chromosomal localization.. https://doi.org/10.1073/pnas.91.26.13023

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