VASE exon expression alters NCAM-mediated cell-cell interactions.

A Chen; S Haines; K Maxson; R A Akeson

doi:10.1002/jnr.490380502

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VASE exon expression alters NCAM-mediated cell-cell interactions.

A Chen·S Haines·K Maxson·R A Akeson

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Abstract

The neural cell adhesion molecule (NCAM) is found on cells as several related polypeptides formed by alternative splicing of the single NCAM gene. The alternatively spliced 30-bp VASE exon in the fourth immunoglobulin-like domain is the structural variation nearest those portions of the polypeptide proposed to mediate cell-cell adhesion. To test the ability of distinct forms of the NCAM molecules to mediate cell adhesion, L cells were transfected with expression vectors encoding rat 140 kD NCAM +/- the VASE exon. L cell lines which expressed these polypeptides were isolated and tested for self-aggregation in a low shear, rapid aggregation assay. Increased cellular aggregation of the transfectants was observed to be a function of the NCAM molecule expressed. These transfected cells showed segregation in a long term co-aggregation assay: cells expressing NCAM--VASE formed aggregates which tended to exclude cells expressing NCAM+VASE and vice versa. These results provide direct evidence that this small difference in NCAM structure is sufficient to allow segregation of cells.

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Animals Cell Adhesion Cell Adhesion Molecules, Neuronal Cell Aggregation Exons L Cells Mice Protein Structure, Tertiary RNA Splicing Rats Recombinant Fusion Proteins Transfection Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

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A Chen, S Haines, K Maxson, R A Akeson. 1994-08-01. VASE exon expression alters NCAM-mediated cell-cell interactions.. https://doi.org/10.1002/jnr.490380502

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