Efficient optimization of ELISAs.

S R Reiken; B J Van Wie; H Sutisna; D L Kurdikar; W C Davis

doi:10.1016/0022-1759(94)90157-0

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Efficient optimization of ELISAs.

S R Reiken·B J Van Wie·H Sutisna·D L Kurdikar·W C Davis

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Abstract

In this paper, we describe a technique for using the statistical method of fractional factorial design in the optimization of an ELISA. Fractional factorial design dramatically reduces the total number of experiments required in the optimization. In addition, this technique enables us to determine the parameters that give the maximum sensitivity range with the most accuracy for the ELISA studied.

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Animals Antibodies, Monoclonal Dose-Response Relationship, Immunologic Enzyme-Linked Immunosorbent Assay Rats Receptors, Nicotinic Statistics as Topic Journal Article Research Support, Non-U.S. Gov't

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S R Reiken, B J Van Wie, H Sutisna, D L Kurdikar, W C Davis. 1994-12-28. Efficient optimization of ELISAs.. https://doi.org/10.1016/0022-1759(94)90157-0

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