Constrained peptide libraries as a tool for finding mimotopes.

S J McConnell; M L Kendall; T M Reilly; R H Hoess

doi:10.1016/0378-1119(94)90640-8

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Constrained peptide libraries as a tool for finding mimotopes.

S J McConnell·M L Kendall·T M Reilly·R H Hoess

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Abstract

The monoclonal antibody (mAb) KAA8 recognizes the peptide angiotensin II (AII). The KAA8 mAb was biopanned using two phage-displayed peptide libraries, one unconstrained, the other constrained by a disulfide bond. After several cycles of biopanning, both libraries showed enrichment for phage that bind KAA8. Phage isolated from the unconstrained library contain a consensus sequence that matches the sequence of AII. A consensus sequence was also identified from the constrained library that does not resemble the AII sequence, and represents a mimotope of AII. We have also demonstrated that monovalent phage display can be used to discriminate between modest and high-affinity binding peptides.

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Amino Acid Sequence Angiotensin II Antibodies, Monoclonal Cloning, Molecular Cosmids Gene Library Molecular Sequence Data Peptides Comparative Study Journal Article

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S J McConnell, M L Kendall, T M Reilly, R H Hoess. 1994-12-30. Constrained peptide libraries as a tool for finding mimotopes.. https://doi.org/10.1016/0378-1119(94)90640-8

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