[Plasmid from Halobacterium halobium and its restriction map].

Abstract

In this paper, ten strains of H. halobium were screened for the occurrence of plasmid by four methods. Six of the strains harbored plasmids of high molecular weight. Of all the detecting methods, the TENS method was the best for H. halobium. J7 strain contains only one type of plasmid named as pHH205 which showed stationary CCC form. The highest copy number of the plasmid was observed in late stationary growth phase, which was approximately 49 copies per cell. The molecular weight is 16.7kb. The sites of five endonucleases had been determined for pHH205 and three of them, namely BamHI, HindIII and XbaI, were single sites neighboring to each other.