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Insertion sequence IS2 near the gene for prophage lambda excision.

E Mosharrafa·W Pilacinski·J Zissler·M Fiandt·W Szybalski
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Abstract

In this study we characterize a variant of the lambdacI857S7 prophage, designated lambdabi2cI857S7, which carries a DNA insertion. The insertion sequence is IS2, and it resides in the antipolar orientation II just upstream from the gene for prophage excision (xis) at 61.6%lambda. This bi2 insertion mutant could prove valuable for studies on possible recombination functions of IS2 DNA and of its effect on the lambda integration and excision functions.

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Chromosome MappingChromosomes, BacterialColiphagesDNA, ViralElectrophoresis, Agar GelEscherichia coliMicroscopy, ElectronNucleic Acid ConformationJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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E Mosharrafa, W Pilacinski, J Zissler, M Fiandt, W Szybalski. 1976-08-10. Insertion sequence IS2 near the gene for prophage lambda excision.. https://doi.org/10.1007/bf00337943

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