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Intracisternal A-type particle elements as genetic markers: detection by repeat element viral element amplified locus-PCR.

N Kaushik·J P Stoye
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Abstract

We describe a novel, PCR-based technique termed REVEAL-PCR for examining the inheritance of intracisternal A-type particles (IAP). Amplifications use an unlabeled primer to SINE repeats and a radiolabeled primer to the IAP long terminal repeat; labeled products, which can be resolved on sequencing gels, are formed when IAPs lie in proximity to SINEs. With this technique we have identified a total of 124 polymorphisms in the BXH and CXS recombinant inbred strains. We suggest that this method will be equally applicable for examining other gene families present at around a thousand copies per genome.

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AnimalsBase SequenceFeasibility StudiesGenes, Intracisternal A-ParticleGenetic MarkersLeukemia Virus, MurineMiceMice, Inbred BALB CMice, Inbred C3HMice, Inbred C57BLMolecular Sequence DataPolymerase Chain ReactionProvirusesRepetitive Sequences, Nucleic AcidJournal ArticleResearch Support, Non-U.S. Gov't

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N Kaushik, J P Stoye. 1994. Intracisternal A-type particle elements as genetic markers: detection by repeat element viral element amplified locus-PCR.. https://doi.org/10.1007/bf00426074

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