Murine M290 integrin expression modulated by mast cell activation.

T J Smith; L A Ducharme; S K Shaw; C M Parker; M B Brenner; P J Kilshaw; J H Weis

doi:10.1016/1074-7613(94)90070-1

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Murine M290 integrin expression modulated by mast cell activation.

T J Smith·L A Ducharme·S K Shaw·C M Parker·M B Brenner·P J Kilshaw·J H Weis

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Abstract

Intraepithelial T lymphocytes possess a (TGF beta)-inducible integrin complex that consists of the beta 7 integrin chain in association with a novel alpha chain, murine alpha M290. Using antibodies directed against the murine alpha M290 chain, IL-3-dependent murine bone marrow-derived mast cells demonstrated positive staining after induction with TGF beta. Sequence information from the human intraepithelial T lymphocyte-alpha E gene sequence was used to clone the murine homolog from a TGF beta-induced T cell library. The murine alpha E homolog gene encodes a protein recognized by antibodies to alpha M290. Expression of this gene by bone marrow-derived mast cells was found to be induced by TGF beta or IgE-mediated cross-linking of Fc epsilon R1. Furthermore, exposure of murine TK-1 cells to supernatants from activated mast cells induces the expression of the alpha M290 gene, indicating that a mast cell mediator(s) can act in a paracrine manner to induce expression of this integrin complex by other cells.

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Amino Acid Sequence Animals Base Sequence Cell Degranulation Gene Expression Humans Immunoglobulin E Integrins Mast Cells Mice Mice, Inbred BALB C Molecular Sequence Data Molecular Structure Proteins Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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T J Smith, L A Ducharme, S K Shaw, C M Parker, M B Brenner, P J Kilshaw, J H Weis. 1994. Murine M290 integrin expression modulated by mast cell activation.. https://doi.org/10.1016/1074-7613(94)90070-1

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