Techniques in mammalian genome mapping.

L C Schalkwyk; F Francis; H Lehrach

doi:10.1016/0958-1669(95)80007-7

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Techniques in mammalian genome mapping.

L C Schalkwyk·F Francis·H Lehrach

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Abstract

Increasing emphasis is being given to genomic cloning using Escherichia coli vectors of intermediate insert capacity, such as bacteriophage P1, P1-derived artificial chromosomes and the F factor based bacterial artificial chromosomes. These vectors are being used in addition to yeast artifical chromosomes (YACs) in recognition of the difficulties encountered with YAC stability and with handling of YAC DNAs (problems that will not easily be overcome). Nonetheless, YACs remain the most practical cloning system for global contig building. Efforts are currently under way to produce YAC contigs that represent the human and mouse genomes, and these will increasingly exploit extensive anchoring to detailed genetic maps. Intermediate capacity clone collections based on YAC contigs will follow, enabling the compilation of mapped gene catalogues. In this way, the era of big gene hunts will draw to a close.

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Animals Bacteriophage P1 Chromosome Mapping Chromosomes, Artificial, Yeast Cloning, Molecular Escherichia coli Genetic Vectors Genome Genome, Human Humans Mice Polymerase Chain Reaction Journal Article Review

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L C Schalkwyk, F Francis, H Lehrach. 1995. Techniques in mammalian genome mapping.. https://doi.org/10.1016/0958-1669(95)80007-7

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