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PubMed · 7894654

Microdialysis sampling coupled on-line to fast microbore liquid chromatography.

A Chen·C E Lunte
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Abstract

A system which combines intravenous microdialysis sampling on-line with fast microbore liquid chromatographic analysis is described. Using a 14 mm x 1 mm I.D. ODS column, caffeine, theobromine and paraxanthine could be resolved in under 1 min and acetaminophen and its two main metabolites, the glucuronide and sulfate conjugates, could be resolved in 30 s. This fast separation allowed on-line microdialysis sampling to be conducted with a 1-min sampling interval. The system was evaluated by monitoring the pharmacokinetics following intravenous administration of acetaminophen and caffeine.

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AcetaminophenAnimalsCaffeineChromatography, High Pressure LiquidMaleMicrodialysisRatsRats, Sprague-DawleyTheobromineTheophyllineJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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A Chen, C E Lunte. 1995-02-03. Microdialysis sampling coupled on-line to fast microbore liquid chromatography.. https://doi.org/10.1016/0021-9673(94)00634-l

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