DNA amplification fingerprinting using arbitrary oligonucleotide primers.

G Caetano-Anollés; B J Bassam

doi:10.1007/bf02788052

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DNA amplification fingerprinting using arbitrary oligonucleotide primers.

G Caetano-Anollés·B J Bassam

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Abstract

DNA amplification fingerprinting (DAF) is a strategy for genetic typing and mapping that uses one or more very short (> or = 5 nt) arbitrary oligonucleotides to direct the enzymatic amplification of discrete portions of a DNA template resulting in a spectrum of products characteristic of the DNA starting material. Polymorphisms from simple banding patterns are useful as genetic markers while more complex and informative patterns are suitable for DNA fingerprinting. The use of polyacrylamide gel electrophoresis and silver staining can adequately resolve the spectrum of DAF products into detailed and reproducible patterns.

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Base Sequence DNA Fingerprinting DNA Primers Gene Amplification Molecular Sequence Data Polymorphism, Restriction Fragment Length Journal Article

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G Caetano-Anollés, B J Bassam. DNA amplification fingerprinting using arbitrary oligonucleotide primers.. https://doi.org/10.1007/bf02788052

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