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Changes in homeobox-containing gene expression during ectopic bone formation induced by bone morphogenetic protein.

T Iimura·S Oida·K Takeda·Y Maruoka·S Sasaki
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Abstract

Expression of homeobox genes in relation to ectopic bone formation induced by bone morphogenetic protein (BMP) was investigated. Oligonucleotide primers corresponding to highly conserved regions of Hox cluster and Msx genes were designed to detect homeobox sequences by means of the polymerase chain reaction (PCR). Nine rat homologues of Hox cluster genes and two Msx genes were discovered in the BMP-implanted tissue, at earlier stage and later cartilage and bone formation stage, respectively. The PCR study provided evidence of dynamic changes in BMP-induced homeobox gene expression.

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Amino Acid SequenceAnimalsBase SequenceBone DevelopmentBone Morphogenetic ProteinsCartilageChoristomaCloning, MolecularCollagenConserved SequenceDNA PrimersDrug ImplantsGene Expression RegulationGenes, HomeoboxHumansMolecular Sequence DataMultigene FamilyOsteoblastsPolymerase Chain ReactionProteinsRatsRats, WistarSequence Homology, Nucleic AcidComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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T Iimura, S Oida, K Takeda, Y Maruoka, S Sasaki. 1994-06-15. Changes in homeobox-containing gene expression during ectopic bone formation induced by bone morphogenetic protein.. https://doi.org/10.1006/bbrc.1994.1798

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