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PubMed · 791366

Study on the surface polypeptides isolated from human lymphoblastoid ce-ls by ionic shock.

E H Lo·M H Ng·W S Ng·S Lui·H C Ho
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Abstract

Ionic shock treatment in the presence of 10% glycerol is an efficient and selective method for extracting cell surface components from Raji cells and effecting the solubilization of up to 22 polypeptides. The majority of these shock-released polypeptides are accessible to lactoperoxidase radioiodination. Sera from rabbits immunized against these soluble extracts are reactive against Raji cell surface as indicated by indirect membrane immunofluorescence and agglutination assays.

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Agglutination TestsCell MembraneFluorescent Antibody TechniqueHumansLactoperoxidaseLymphocytesMolecular WeightPeptidesSubcellular FractionsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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E H Lo, M H Ng, W S Ng, S Lui, H C Ho. 1976-10-22. Study on the surface polypeptides isolated from human lymphoblastoid ce-ls by ionic shock.. https://doi.org/10.1016/0304-4165(76)90332-9

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