Isolation, sequence analysis and characterization of a cDNA encoding human chaperonin 10.

J J Chen; D J McNealy; S Dalal; E J Androphy

doi:10.1016/0167-4781(94)90268-2

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Isolation, sequence analysis and characterization of a cDNA encoding human chaperonin 10.

J J Chen·D J McNealy·S Dalal·E J Androphy

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Abstract

A full-length cDNA clone encoding chaperonin 10 (cpn10) from a HeLa cell cDNA library was isolated. The cDNA is 538 bp in length, contains an ATG codon and a putative polyadenylation signal, and specifies a protein of 102 amino acids. Immunoprecipitation experiment showed that this human cpn10 has an apparent molecular mass of 11 kDa in sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

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Amino Acid Sequence Base Sequence Chaperonin 10 Cloning, Molecular DNA, Complementary HeLa Cells Heat-Shock Proteins Humans Molecular Sequence Data Regulatory Sequences, Nucleic Acid Sequence Analysis, DNA Sequence Homology, Amino Acid Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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J J Chen, D J McNealy, S Dalal, E J Androphy. 1994-09-13. Isolation, sequence analysis and characterization of a cDNA encoding human chaperonin 10.. https://doi.org/10.1016/0167-4781(94)90268-2

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