Nicotinic cholinergic system involvement in eyeblink classical conditioning in rabbits.

D S Woodruff-Pak; Y T Li; A Kazmi; W R Kem

doi:10.1037//0735-7044.108.3.486

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Nicotinic cholinergic system involvement in eyeblink classical conditioning in rabbits.

D S Woodruff-Pak·Y T Li·A Kazmi·W R Kem

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Abstract

A nicotinic cholinergic antagonist, mecamylamine (MEC), was administered to rabbits tested on eyeblink classical conditioning (EBCC) in the 750-ms delay paradigm for 10 90-trial sessions. Nicotinic receptors were measured in 3 brain regions in 5 treatment groups: paired conditioned stimulus-unconditioned stimulus (CS-US) presentations with (a) vehicle, young; (b) vehicle, older; (c) 0.5 mg/kg MEC, young; unpaired CS-US with (d) 0.5 mg/kg MEC, young; and (e) vehicle, young. Daily MEC injections disrupted acquisition in young rabbits (769 trials to learning criterion vs. 323 trials for vehicle-treated young rabbits). MEC-treated young rabbits learned similarly to older rabbits. Brain nicotinic receptors were not affected by 10 daily MEC injections. To our knowledge, this experimental protocol, using a low MEC dose to selectively inhibit nicotinic cholinergic receptors, is the first to demonstrate a role for nicotinic cholinergic receptors in EBCC.

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Animals Association Learning Brain Brain Mapping Conditioning, Classical Conditioning, Eyelid Female Male Mecamylamine Mental Recall Rabbits Receptors, Cholinergic Receptors, Nicotinic Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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D S Woodruff-Pak, Y T Li, A Kazmi, W R Kem. 1994. Nicotinic cholinergic system involvement in eyeblink classical conditioning in rabbits.. https://doi.org/10.1037/%2F0735-7044.108.3.486

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