A consensus Alu repeat probe for physical mapping.

M A Batzer; M Alegria-Hartman; P L Deininger

doi:10.1016/1050-3862(94)90058-2

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A consensus Alu repeat probe for physical mapping.

M A Batzer·M Alegria-Hartman·P L Deininger

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Abstract

Physical mapping of the human genome involves a variety of complex hybridization-based procedures, some of which rely upon the ability to separate human clones derived from human-rodent hybrid cell lines from those that contain background rodent-derived DNA sequences. The ability to block the repetitive element (Alu repeat) portion of inter-Alu PCR products derived from a variety of complex sources is also crucial for the isolation of unique DNA sequences. Here we report the construction and characterization of a new consensus Alu repeat probe (pPD39) designed for these purposes.

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Animals Base Sequence Chromosome Mapping Consensus Sequence DNA Primers DNA Probes Genetic Techniques Genome, Human Humans Hybrid Cells Indicators and Reagents Molecular Sequence Data Nucleic Acid Hybridization Polymerase Chain Reaction Repetitive Sequences, Nucleic Acid Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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M A Batzer, M Alegria-Hartman, P L Deininger. 1994. A consensus Alu repeat probe for physical mapping.. https://doi.org/10.1016/1050-3862(94)90058-2

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