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Relationships between molecular interactions (nucleotides, lipids and proteins) and structural features of the bovine brain 21-kDa protein.

S Bucquoy·P Jollès·F Schoentgen
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Abstract

A cytosolic 21-kDa protein isolated from bovine brain was demonstrated to bind hydrophobic ligands, particularly phosphatidylethanolamine. It was encountered in several tissues and species; however, its accurate function remained partially unknown. In order to obtain information from its structural features, we built a molecular model which revealed it to possess a nucleotide-binding site. In the present research, we describe the affinity of the bovine brain 21-kDa protein for nucleotides, and its association with cytosolic proteins, small GTP-binding proteins and lipid droplets. Our results suggest that, through its association with small GTP-binding proteins, the 21-kDa protein is implicated in signal mechanisms during cell growth and maturation.

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Amino Acid SequenceAnimalsBrainCattleCytosolGTP-Binding ProteinsHumansIn Vitro TechniquesLipid MetabolismModels, MolecularMolecular Sequence DataMolecular StructureMolecular WeightNerve Tissue ProteinsNucleotidesPhosphatidylethanolaminesProtein ConformationRatsSequence Homology, Amino AcidSignal TransductionJournal ArticleResearch Support, Non-U.S. Gov't

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S Bucquoy, P Jollès, F Schoentgen. 1994-11-01. Relationships between molecular interactions (nucleotides, lipids and proteins) and structural features of the bovine brain 21-kDa protein.. https://doi.org/10.1111/j.1432-1033.1994.1203b.x

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