Decrease of heat shock protein 27/28 with heat stress in HTLV-I-transformed cells.

D Sun; J J Sauk; D W Archibald

doi:10.1006/exmp.1994.1014

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PubMed · 7957775

Decrease of heat shock protein 27/28 with heat stress in HTLV-I-transformed cells.

D Sun·J J Sauk·D W Archibald

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Abstract

We have previously reported an increase of human T-lymphotropic leukemia/lymphoma virus type I (HTLV-I) replication after heat treatment of MT-2 cells, an HTLV-I-transformed human lymphoid cell line. In this study, we investigated the effect of heat stress on the expression of Hsp27/28 in MT-2 cells. In contrast with previous studies of other cell types, a decrease of Hsp27/28 expression in MT-2 cells and an increase of Hsp70 family proteins in both MT-2 and uninfected lymphoid CEM cells were found following heat treatment at 42 degrees C. Furthermore, heat treatment resulted in an early rapid increase in the phosphorylated form of Hsp27/28 in both MT-2 and CEM cells. The results suggest that early post-translational phosphorylation of HSP27/28 could be a determinant of the ability of MT-2 cells to survive hyperthermia.

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Blotting, Northern Blotting, Western Cell Line, Transformed Cell Transformation, Neoplastic Cell Transformation, Viral Electrophoresis, Polyacrylamide Gel Heat-Shock Proteins Hot Temperature Human T-lymphotropic virus 1 Humans Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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D Sun, J J Sauk, D W Archibald. 1994. Decrease of heat shock protein 27/28 with heat stress in HTLV-I-transformed cells.. https://doi.org/10.1006/exmp.1994.1014

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