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PubMed · 7961815

Magnesium-induced structural changes in tubulin.

A Bhattacharya·B Bhattacharyya·S Roy
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Abstract

Exogenously added Mg2+, in the intracellular concentration range, has significant effect on the conformation of tubulin as monitored by tryptophan fluorescence. This magnesium-induced conformational change is reflected in the change of the slope and intercept of the Lehrer plot. The off-rate of colchicine from colchicine-tubulin complex is also decreased severalfold upon the addition of exogenous magnesium. The conformational change is magnesium-specific, and magnesium can only be replaced by manganese. GTP, CaCl2, and NaCl have no effect. Magnesium, however has no effect on tryptophan accessibilities of tubulin-S. Implications of these results are discussed in terms of C-terminal tail-body interaction.

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AnimalsBinding SitesFluorescenceGoatsMagnesiumMicroscopy, ElectronMicrotubulesNucleotidesProtein ConformationTubulinJournal ArticleResearch Support, Non-U.S. Gov't

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A Bhattacharya, B Bhattacharyya, S Roy. 1994-11-18. Magnesium-induced structural changes in tubulin.. https://pubmed.ncbi.nlm.nih.gov/7961815/

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