PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 7971165

Responses of endothelial cells to hypotonic solutions: lack of regulatory volume decrease.

P De Smet·M Oike·G Droogmans·W Van Driessche·B Nilius
View original source ↗Export metadata ↓

Abstract

Hypotonic stress (HTS) activates a Cl- current and releases intracellular Ca2+ in vascular endothelial cells, but there is no co-activation of K(+)-channels. The concomitant increase in cell volume, as assessed from the changes in endothelial cell thickness, is not followed by a regulatory volume decrease (RVD). This lack compensation of the volume increase may be explained by the absence of a concomitant activation of a K+ outward current, resulting in an insufficient efflux of osmolytes during HTS.

Explore related subjects

Cell SizeChloride ChannelsEndothelium, VascularFemaleFura-2HumansHypotonic SolutionsIn Vitro TechniquesPatch-Clamp TechniquesPregnancyUmbilical VeinsJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

P De Smet, M Oike, G Droogmans, W Van Driessche, B Nilius. 1994. Responses of endothelial cells to hypotonic solutions: lack of regulatory volume decrease.. https://doi.org/10.1007/bf00374757

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1999

Some relationships between membrane phospholipid domains, conformational order, and cell shape in intact human erythrocytes.

A novel method developed in this laboratory [D.J. Moore et al., Biochemistry 35 (1996) 229-235; D.J. Moore et al., Biochemistry 36 (1997) 660-664] to study the conformational order and the propensity for domain formation of specific phospholipids in intact human erythrocytes is extended to two additional species. Acyl chain perdeuterated 1,2-dilauroylphosphatidylethanolamine (diC12PE-d46) was incorporated preferentially (in separate experiments) into the inner leaflet of stomatocytic erythrocytes and into the outer leaflet of echinocytic erythrocytes, while acyl chain perdeuterated 1,2-dipentadecanoylphosphatidylcholine (diC15PC-d58) was incorporated into the outer leaflet of echinocytic erythrocytes. The conformational order and phase behavior of the incorporated molecules were monitored through FT-IR studies of the temperature dependence of the CD2 stretching vibrations. For both diC12PE-d46 and diC15PC-d58, the gel-->liquid crystal phase transition persisted when these lipids were located in the outer leaflet of echinocytic cells, a result indicative of the persistence of phospholipid domains. In each case, the transition widths were broadened compared to the pure lipids, suggestive of either small domains or the presence of additional molecular components within the domains. The conformational order of diC12PE-d46 differed markedly depending on its location and the morphology of the cells. When located predominantly in the inner membrane of stomatocytes, the phase transition of this species was abolished and the conformational order compared with pure lipid vesicles at the same temperature was much lower. The current results along with our previous studies provide a sufficient experimental basis to deduce some general principles of phospholipid conformational order and organization in both normal and shape-altered erythrocytes.

Cell Size↗
PubMed1998

Measurement of cell volume loss in the liquid region preceding an advancing phase change interface.

It is well understood that the solidification of a solution results in a redistribution of solute in the liquid zone. For the freezing of suspensions of cells it is anticipated that accumulation of solute in the region leading a growing ice phase will cause an osmotic response in cells before the ice phase reaches the cells. To measure this phenomenon in a specific algal species, the volume changes in Chlorococcum texanum during freezing were studied using directional solidification cryomicroscopy. The relative cell volume was tracked continuously as a function of temperature and position as cells encountered the moving phase front. The loss of cell volume was measured in the liquid region containing concentrated solute ahead of the growing solid phase.

Cell Size↗