Selection of peptides with affinity for single stranded DNA using a phage display library.

M Krook; K Mosbach; C Lindbladh

doi:10.1006/bbrc.1994.2537

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Selection of peptides with affinity for single stranded DNA using a phage display library.

M Krook·K Mosbach·C Lindbladh

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Abstract

A hexapeptide phage library was used to affinity select peptides which interact with single stranded heptaoligonucleotides consisting of cytosine (oligo-C). Selections were performed in two different buffer systems, 50 mM 2[N-Morpholino]ethanesulphonic acid(MES)-buffer, pH 5.5, and 50 mM Tris(hydroxymethyl)-aminomethane, 150 mM NaCl, pH 7.5, respectively. Selection was more successful in 50 mM MES-buffer and three clones with affinity for oligo-C were further investigated. The peptides, Pro-Pro-Pro-Leu-Tyr-Phe, Arg-Phe-Cys-Asp-Thr-Ser and Arg-Ser-Arg-Leu-Ile-Trp, all interacted more strongly with oligo-C compared to the original peptide phage library and wildtype phage. The selected clones also showed different specificity in the interaction with oligo-C, -G, -A and -T.

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Amino Acid Sequence Binding Sites Coliphages DNA, Single-Stranded DNA, Viral Molecular Sequence Data Peptides Journal Article Research Support, Non-U.S. Gov't

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M Krook, K Mosbach, C Lindbladh. 1994-10-28. Selection of peptides with affinity for single stranded DNA using a phage display library.. https://doi.org/10.1006/bbrc.1994.2537

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