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Crystallization and preliminary X-ray diffraction studies on ADP-ribosylation factor 1.

S Greasley·H Jhoti·A C Fensome·S Cockcroft·G M Thomas·B Bax
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Abstract

ADP-ribosylation factor 1 (ARF-1) is a member of a family of small G-proteins that regulate both intracellular vesicle transport and phospholipase D activity. Crystals of ARF-1 suitable for X-ray diffraction analysis have been grown in the presence of GDP by the hanging drop vapour diffusion method. Crystals grow in space group C2 with cell dimensions a = 122.36 A, b = 45.01 A, c = 91.96 A and beta = 133.62 degrees and diffract to at least 2.3 A resolution. A second crystal form has been characterized (space group C2, a = 69.70 A, b = 45.25 A, c = 60.45 A, beta = 109.6 degrees) but does not grow reproducibly.

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ADP-Ribosylation FactorsCarrier ProteinsCrystallizationCrystallography, X-RayGTP-Binding ProteinsJournal Article

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S Greasley, H Jhoti, A C Fensome, S Cockcroft, G M Thomas, B Bax. 1994-12-16. Crystallization and preliminary X-ray diffraction studies on ADP-ribosylation factor 1.. https://doi.org/10.1006/jmbi.1994.1759

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