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PubMed · 7997882

Specification of C/EBP function during Drosophila development by the bZIP basic region.

P Rørth
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Abstract

The biologically relevant interactions of a transcription factor are those that are important for function in the organism. Here, a transgenic rescue assay was used to determine which molecular functions of Drosophila CCAAT/enhancer binding protein (C/EBP), a basic region-leucine zipper transcription factor, are required for it to fulfill its essential role during development. Chimeric proteins that contain the Drosophila C/EBP (DmC/EBP) basic region, a heterologous zipper, and a heterologous activation domain could functionally substitute for DmC/EBP. Mammalian C/EBPs were also functional in Drosophila. In contrast, 9 of 25 single amino acid substitutions in the basic region disrupted biological function. Thus, the conserved basic region specifies DmC/EBP activity in the organism.

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Amino Acid SequenceAnimalsAnimals, Genetically ModifiedBase SequenceBasic-Leucine Zipper Transcription FactorsCCAAT-Enhancer-Binding ProteinsDNADNA-Binding ProteinsDrosophilaFemaleG-Box Binding FactorsLeucine ZippersMaleMolecular Sequence DataNuclear ProteinsRecombinant Fusion ProteinsTranscription FactorsTranscriptional ActivationJournal ArticleResearch Support, Non-U.S. Gov't

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P Rørth. 1994-12-16. Specification of C/EBP function during Drosophila development by the bZIP basic region.. https://doi.org/10.1126/science.7997882

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