Rabbit knee-joint meniscal proteoglycans.

N Heise; O M Toledo

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PubMed · 8032316

Rabbit knee-joint meniscal proteoglycans.

N Heise·O M Toledo

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Abstract

A major proteoglycan (PG) in rabbit meniscal fibrocartilage was extracted with 4 M guanidine-HCl. It represented 63% of the total uronic acid originally present in the tissue. The non-extractable PG could be released by treating the guanidine HCl-extracted tissue with trypsin followed by alkaline hydrolysis. The glycosaminoglycan (GAG) chains from the non-extractable PGs are shown to be of a different type than the extractable forms since the relative amounts of chondroitin sulphate isomers differ from each other.

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Animals Carbohydrate Conformation Carbohydrate Sequence Chondroitinases and Chondroitin Lyases Disaccharides Female Glycosaminoglycans Guanidine Guanidines Joints Male Molecular Sequence Data Proteoglycans Rabbits Trypsin Journal Article Research Support, Non-U.S. Gov't

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N Heise, O M Toledo. 1994. Rabbit knee-joint meniscal proteoglycans.. https://pubmed.ncbi.nlm.nih.gov/8032316/

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