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PubMed · 8041629

Sequencing of oligonucleotide phosphorothioates based on solid-supported desulfurization.

T K Wyrzykiewicz·D L Cole
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Abstract

We described a solid-supported desulfurization procedure allowing easy access to the sequence analysis of oligonucleotide phosphorothioates. The described method is based upon selective removal of the 2-cyanoethyl phosphate protecting groups, followed by iodine-promoted desulfurization of the resulting phosphorothioate diesters. Automatic oxidation of oligonucleotide phosphorothioates, anchored via an ester linkage to a standard solid support (LCAA/CPG), is combined with Maxam-Gilbert solid-support sequencing. The overall procedure allows rapid simultaneous sequence analysis of several oligonucleotide analogs.

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Base SequenceDNAMolecular Sequence DataOligonucleotidesOrganothiophosphatesSequence Analysis, DNASulfurThionucleotidesJournal Article

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T K Wyrzykiewicz, D L Cole. 1994-07-11. Sequencing of oligonucleotide phosphorothioates based on solid-supported desulfurization.. https://doi.org/10.1093/nar%2F22.13.2667

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