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PubMed · 80457

Secondary in vitro generation of cytolytic T-lymphocytes (CTL's) in the murine sarcoma virus system. Virus-specific CTL induction across the H-2 barrier.

D Collavo·A Parenti·G Biasi·L Chieco-Bianchi·A Colombatti
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Abstract

Following in vitro stimulation of murine sarcoma virus Moloney isolate (M-MuSV)-immune spleen cells with syngeneic antigenically related Moloney leukemia cells, highly efficient cytotoxic T-lymphocytes (CTL's) were generated. The cytotoxic effect was directed only against H-2-compatible target cells bearing M-MuSV tumor-associated antigens (TAA). However, in a cold target competition assay a weak but detectable capacity to block CTL activity was also obtained when allogeneic Moloney leukemia cells were added. Moreover, when M-MuSV-immune spleen cells from mice inoculated with virus 14 days previously were stimulated by allogeneic Moloney leukemia cells, a strong cytotoxic effect toward syngeneic and allogeneic tumor cells bearing M-MuSV TAA was elicited.

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AnimalsAntigens, NeoplasmAntigens, ViralCytotoxicity, ImmunologicEpitopesFemaleH-2 AntigensIn Vitro TechniquesLeukemia, ExperimentalMaleMiceMice, Inbred BALB CMice, Inbred C57BLMoloney murine leukemia virusSarcoma, ExperimentalT-LymphocytesTumor Virus InfectionsJournal Article

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D Collavo, A Parenti, G Biasi, L Chieco-Bianchi, A Colombatti. 1978. Secondary in vitro generation of cytolytic T-lymphocytes (CTL's) in the murine sarcoma virus system. Virus-specific CTL induction across the H-2 barrier.. https://pubmed.ncbi.nlm.nih.gov/80457/

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