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Rapid, nonradioactive, and quantitative method to analyze zona pellucida modifications in single mouse eggs.

J Moos·P Kalab·G S Kopf·R M Schultz
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Abstract

A rapid, nonradioactive method to monitor the ZP2 to ZP2f conversion in the zona pellucida of single mouse eggs has been developed. This assay is based on the chemiluminescent detection of biotinylated ZP2 and ZP2f following electrophoresis under reducing conditions and electrophoretic transfer to Immobilon P. This method is about 10 times faster and detects similar extents of ZP2 to ZP2f conversion following A23187-induced egg activation, when compared to the commonly used radioiodination procedures.

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AnimalsAutoradiographyBiotinBlotting, WesternEgg ProteinsElectrophoresis, Gel, Two-DimensionalEvaluation Studies as TopicFemaleIn Vitro TechniquesIodine RadioisotopesLuminescent MeasurementsMembrane GlycoproteinsMethodsMiceReceptors, Cell SurfaceZona PellucidaZona Pellucida GlycoproteinsComparative StudyJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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J Moos, P Kalab, G S Kopf, R M Schultz. 1994. Rapid, nonradioactive, and quantitative method to analyze zona pellucida modifications in single mouse eggs.. https://doi.org/10.1002/mrd.1080380115

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