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PubMed · 8059941

A method for protein assay in Laemmli buffer.

J O Karlsson·K Ostwald·C Kåbjörn·M Andersson
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Abstract

A simple, cheap, and rapid assay for soluble and membrane-bound protein solubilized in Laemmli sodium dodecyl sulfate sample buffer is described. Following the addition of trichloroacetic acid to a final concentration of 24%, the induced turbidity was measured at 570 nm in a microplate reader, after an incubation period of approximately 10-30 min. The assay is suitable for measuring bovine serum albumin at a concentration of 15 to 500 micrograms/ml in Laemmli buffer, corresponding to 2 to 75 micrograms protein per well on the microtiter plate. Other nonproteinaceous macromolecules in the sample may introduce an error.

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AnimalsBrain ChemistryIndicators and ReagentsNephelometry and TurbidimetryNerve Tissue ProteinsProteinsRabbitsSensitivity and SpecificitySerum Albumin, BovineSodium Dodecyl SulfateTromethamineComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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J O Karlsson, K Ostwald, C Kåbjörn, M Andersson. 1994-05-15. A method for protein assay in Laemmli buffer.. https://doi.org/10.1006/abio.1994.1243

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