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Selective suppression of IgG2a subclass in LEC rats during development.

Y Ikeda·T Sugiyama·M Takahashi·N Taniguchi
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Abstract

The LEC rat is a mutant strain that has been established as a model of hepatitis and hepatoma. In addition to hepatic disorders, it has been found that this strain has a defect in T cell maturation, and has low levels of serum IgG. Sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot analysis showed that low levels of serum IgG were largely due to reduction of the IgG2a subclass. Quantitative determination of IgG subclasses by enzyme-linked immunosorbent assay (ELISA) using subclass-specific antibodies indicated that IgG2b and IgG2c subclasses were increased during development in this strain, whereas the IgG2a subclass was markedly decreased. These results suggest that dysfunction of some helper T cells in LEC rats selectively suppress synthesis of the IgG2a subclass during development but not affect production of IgG2b and IgG2c.

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AnimalsBlotting, WesternElectrophoresis, Polyacrylamide GelEnzyme-Linked Immunosorbent AssayIgG DeficiencyImmunoglobulin GRatsRats, Mutant StrainsT-LymphocytesJournal ArticleResearch Support, Non-U.S. Gov't

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Y Ikeda, T Sugiyama, M Takahashi, N Taniguchi. 1994-08-18. Selective suppression of IgG2a subclass in LEC rats during development.. https://doi.org/10.1016/0304-4165(94)90168-6

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