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Complementary DNA sequence encoding the major neural cell adhesion molecule isoform in a human small cell lung cancer cell line.

S Saito·Y Tanio·I Tachibana·S Hayashi·T Kishimoto·I Kawase
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Abstract

The neural cell adhesion molecule (N-CAM), a member of the immunoglobulin gene super-family mediating homophilic cell-cell adhesion in a neuroendocrine system, is preferentially expressed in human small cell lung cancer (SCLC). Immunoprecipitation of a panel of SCLC cell lines by monoclonal antibodies (mAbs) specific for N-CAM detects mainly the 145-kDa isoform. This result was correlated with Northern blotting where a single 6.2-kb mRNA was detected in nine SCLC cell lines. To determine cDNA sequence encoding the N-CAM isoform, we selected several cDNA clones encoding N-CAM isolated from OS2-R, a SCLC cell line established in our laboratory. Based on the analysis of the full-length cDNA obtained from two clones, the sequence of this 145-kDa isoform was shown to be essentially identical to that of the 140-kDa N-CAM isoform of neuroblastoma except for a single base pair changed at position 1620 without changing amino acid encoded.

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Amino Acid SequenceBase SequenceBlotting, NorthernCarcinoma, Small CellCell Adhesion Molecules, NeuronalDNA, ComplementaryHumansLung NeoplasmsMolecular Sequence DataTumor Cells, CulturedJournal ArticleResearch Support, Non-U.S. Gov't

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S Saito, Y Tanio, I Tachibana, S Hayashi, T Kishimoto, I Kawase. 1994. Complementary DNA sequence encoding the major neural cell adhesion molecule isoform in a human small cell lung cancer cell line.. https://doi.org/10.1016/0169-5002(94)90660-2

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