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PubMed · 8086434

Intact mummified bone alkaline phosphatase.

U Weser·Y Kaup
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Abstract

Our knowledge to the mode of conservation of mummified structurally and functionally intact biopolymers is limited. Rib samples of a well-preserved 2300-year old ptolemeic mummy were examined whether or not functionally active Zn2Mg alkaline phosphatase could be detected. A protein of M(r) 170 +/- 20 kDa being close to 200 kDa of the enzyme of fresh bones was successfully isolated. Both a 200 kDa protein and a distinct subunit of 60 kDa were seen in SDS-PAGE electrophoresis which was identical to those of fresh bone alkaline phosphatase. There was a significant enzymic activity of 17 mU/mg protein which could be inhibited in the presence of L-homoarginine and 1,10-phenanthroline.

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Alkaline PhosphataseBone and BonesChromatography, GelElectrophoresis, Polyacrylamide GelFemaleHumansMagnesiumMolecular WeightMummiesZincJournal ArticleResearch Support, Non-U.S. Gov't

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U Weser, Y Kaup. 1994-09-21. Intact mummified bone alkaline phosphatase.. https://doi.org/10.1016/0167-4838(94)90177-5

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