Evolutionarily conserved function of CD28 in alpha beta T cell activation.

T P Arstila; O Vainio; O Lassila

doi:10.1111/j.1365-3083.1994.tb03476.x

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PubMed · 8091139

Evolutionarily conserved function of CD28 in alpha beta T cell activation.

T P Arstila·O Vainio·O Lassila

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Abstract

The functional role of the chicken homologue of CD28 was studied. It is expressed on all thymocytes, and both V beta 1- and V beta 2-family expressing peripheral alpha beta T cells. Peripheral gamma delta T cells are CD28-negative. Monoclonal antibody against CD28 had a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MoAb against TCR. V beta 1 and V beta 2 expressing cells responded equally well to stimulation with anti-CD28 in combination with PMA. These responses were resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3. These data indicate a striking conservation of the costimulatory function of CD28 and emphasize the importance of this costimulatory pathway.

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Animals Benzoquinones Biological Evolution CD28 Antigens Cells, Cultured Chickens Cyclosporine Lactams, Macrocyclic Lymphocyte Activation Quinones Receptors, Antigen, T-Cell, alpha-beta Rifabutin T-Lymphocytes Journal Article Research Support, Non-U.S. Gov't

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T P Arstila, O Vainio, O Lassila. 1994. Evolutionarily conserved function of CD28 in alpha beta T cell activation.. https://doi.org/10.1111/j.1365-3083.1994.tb03476.x

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