Differences in the hybridization pattern of Bacillus subtilis genes coding for rDNA depend on the method of DNA preparation.

R N Waterhouse; L A Glover

doi:10.1128/aem.59.3.919-921.1993

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PubMed · 8097621

Differences in the hybridization pattern of Bacillus subtilis genes coding for rDNA depend on the method of DNA preparation.

R N Waterhouse·L A Glover

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Abstract

Three different methods of DNA isolation (organic deproteinization, potassium acetate deproteinization, and the use of cetyltrimethylammonium bromide) have been used to prepare DNA from Bacillus subtilis. Subsequent hybridization with an rDNA probe (DNA coding for rRNA) produces different patterns, which mirror those previously reported to indicate an rDNA deletion.

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Acetates Acetic Acid Bacillus subtilis Cetrimonium Cetrimonium Compounds DNA Probes DNA, Bacterial DNA, Ribosomal Detergents Genes, Bacterial Nucleic Acid Hybridization Polymorphism, Restriction Fragment Length Journal Article Research Support, Non-U.S. Gov't

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R N Waterhouse, L A Glover. 1993. Differences in the hybridization pattern of Bacillus subtilis genes coding for rDNA depend on the method of DNA preparation.. https://doi.org/10.1128/aem.59.3.919-921.1993

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